ABHD10 Polyclonal antibody

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• KD/KO Validated

ABHD10 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22558-1-AP

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 22558-1-AP

  WB result of ABHD10 antibody (22558-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABHD10 transfected HeLa cells.

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  WB analysis using 22558-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22558-1-AP (ABHD10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Y79 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22558-1-AP targets ABHD10 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABHD10 fusion protein Ag18413
• Full Name: abhydrolase domain containing 10
• Calculated Molecular Weight: 306 aa, 34 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC014516
• Gene Symbol: ABHD10
• Gene ID (NCBI): 55347
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABHD10 is a mitochondrial protein that is a member of the APT family. It has 3 isoforms and regulates the S-palmitoylation status of the nucleophilic active site residue of peroxiredoxin-5 (PRDX5) (PMID: 31740833).