ABCD1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ABCD1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18138-1-PBS

Synonyms

EC:3.1.2.-, ATP-binding cassette sub-family D member 1, ATP binding cassette sub family D member 1, ALD, ABCD 1

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Validation Data Gallery

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  WB analysis using 18138-1-AP (same clone as 18138-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18138-1-AP (ABCD1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18138-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 18138-1-AP (same clone as 18138-1-PBS)

  WB result of ABCD1 antibody (18138-1-AP, 1:600) with si-Control and si-ABCD1 transfected HeLa cells.. This data was developed using the same antibody clone with 18138-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 18138-1-AP (same clone as 18138-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18138-1-AP (ABCD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18138-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 18138-1-AP (same clone as 18138-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18138-1-AP (ABCD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18138-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 18138-1-AP (same clone as 18138-1-PBS)

  IP result of anti-ABCD1 (IP:18138-1-AP, 4ug; Detection:18138-1-AP 1:700) with HeLa cells lysate 3000 ug. This data was developed using the same antibody clone with 18138-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 18138-1-AP (same clone as 18138-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18138-1-AP (ABCD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18138-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 18138-1-AP (same clone as 18138-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18138-1-AP (ABCD1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18138-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 18138-1-AP (same clone as 18138-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ABCD1 antibody (18138-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 18138-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 18138-1-AP (same clone as 18138-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ABCD1 (18138-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 18138-1-PBS in a different storage buffer formulation.

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Product Information

18138-1-PBS targets ABCD1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABCD1 fusion protein Ag12757
• Full Name: ATP-binding cassette, sub-family D (ALD), member 1
• Calculated Molecular Weight: 745 aa, 83 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC025358
• Gene Symbol: ABCD1
• Gene ID (NCBI): 215
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.