ABAT Polyclonal antibody

ABAT Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 11349-1-AP

Synonyms

ABAT, GABA aminotransferase, GABA AT, GABA T, GABA transaminase, GABAT, L AIBAT

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Validation Data Gallery

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  WB analysis using 11349-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11349-1-AP (ABAT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 11349-1-AP

  IP Result of anti-ABAT (IP:11349-1-AP, 4ug; Detection:11349-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human pancreas cancer using 11349-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11349-1-AP (ABAT antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

11349-1-AP targets ABAT in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABAT fusion protein Ag1877
• Full Name: 4-aminobutyrate aminotransferase
• Calculated molecular weight: 500 aa, 56 kDa
• Observed molecular weight: 50 kDa
• GenBank accession number: BC015628
• Gene symbol: ABAT
• Gene ID (NCBI): 18
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,IHC	Nat Cell Biol; Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression.; Authors - De Huang
rat	IF	Front Cell Neurosci; Neuronal gamma-aminobutyric acid (GABA) type A receptors undergo cognate ligand chaperoning in the endoplasmic reticulum by endogenous GABA.; Authors - Ping Wang
human	IHC	Cancer Invest; Integrative analysis developing and validating potential candidate biomarkers for cancer stemness features of pan-renal cell carcinoma; Authors - Fei Lin
mouse	WB	Carcinogenesis; EphB6 deficiency in intestinal neurons promotes tumor growth in colorectal cancer by neurotransmitter GABA signaling; Authors - Hao Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Donna (Verified Customer) (11-26-2018)

Works good with minimal background.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HBMEC