|Positive WB detected in||HepG2 cells, mouse brain tissue, rat brain tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11349-1-AP targets ABAT in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ABAT fusion protein Ag1877|
|Full Name||4-aminobutyrate aminotransferase|
|Calculated molecular weight||500 aa, 56 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC015628|
|Gene ID (NCBI)||18|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Nat Cell Biol
Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression.
Front Cell Neurosci
Neuronal gamma-aminobutyric acid (GABA) type A receptors undergo cognate ligand chaperoning in the endoplasmic reticulum by endogenous GABA.
Integrative analysis developing and validating potential candidate biomarkers for cancer stemness features of pan-renal cell carcinoma
EphB6 deficiency in intestinal neurons promotes tumor growth in colorectal cancer by neurotransmitter GABA signaling
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Donna (Verified Customer) (11-26-2018)
Works good with minimal background.