A1CF Polyclonal antibody, PBS Only

A1CF Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25812-1-PBS

Synonyms

APOBEC1-stimulating protein, APOBEC1CF, APOBEC1 complementation factor, ACF65, ACF64

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Validation Data Gallery

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  WB analysis using 25812-1-AP (same clone as 25812-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25812-1-AP (A1CF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25812-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 25812-1-AP (same clone as 25812-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25812-1-AP (A1CF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25812-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 25812-1-AP (same clone as 25812-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25812-1-AP (A1CF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25812-1-PBS in a different storage buffer formulation.

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Product Information

25812-1-PBS targets A1CF in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: A1CF fusion protein Ag22929
• Full Name: APOBEC1 complementation factor
• Calculated Molecular Weight: 594 aa, 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC054873
• Gene Symbol: A1CF
• Gene ID (NCBI): 29974
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

A1CF, also known as Apobec-1 complementation factor, is an RNA-binding protein that plays a crucial role in the post-transcriptional editing of apolipoprotein B (apoB) mRNA. It is a component of the enzyme complex responsible for converting a CAA codon for glutamine to a UAA stop codon in apoB mRNA, resulting in the production of a truncated protein, apoB48, instead of the full-length apoB100.