HRP-conjugated 6*His, His-Tag Antibody 0 Publications

Mouse Monoclonal| Catalog number: HRP-66005

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Freight/Packing

Size: 09µ/gram

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Species specificity:
Recombinant protein

Positive WB detected in:
Recombinant protein

Recommended dilution:
WB : 1:5000-1:50000

Product Information


Source:
Mouse

Purification method:
Protein G Purified

Isotype:
IgG1

Storage:
PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
6xHis Peptide

Full name:
6*His, His-Tag

Calculated molecular weight:
1 kDa

Observed molecular weight:
-

GenBank accession number:

Gene ID (NCBI):

Gene symbol

Synonyms
6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, His tag, HISTIDINE TAGGED, HRP 6XHis, HRP HHHHHH, HRP His tag
Background

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells. This antibody is conjugated with HRP.

Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.


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