SARS-CoV-2 Nucleocapsid Phosphoprotein Recombinant antibody

SARS-CoV-2 Nucleocapsid Phosphoprotein Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

virus

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

8C20

Cat no : 80027-1-RR

Synonyms

nucleocapsid phosphoprotein, sars cov 2, 8C20

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Validation Data Gallery

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  WB analysis of Recombinant protein using 80027-1-RR

  E.coli expressed SARS-CoV-2 Nucleocapsid Phosphoprotein (Cat.NO. Ag30676) was subjected to SDS-PAGE followed by western blot with 80027-1-RR and 80026-1-RR at various work concentration.

• 

  WB analysis of Recombinant protein using 80027-1-RR

  Eukaryotic expressed SARS-CoV-2 Nucleocapsid Phosphoprotein was subjected to SDS-PAGE followed by western blot with 80027-1-RR and 80026-1-RR at various work concentration.

• 

  ELISA experiment of Recombinant protein using 80027-1-RR

  Indirect ELISA was carried out by coating eukaryotic expressed N protein at 70 ng/well followed by blocking and adding serial diluted primary antibody 80026-1-RR and 80027-1-RR respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

• 

  ELISA experiment of Recombinant protein using 80027-1-RR

  Sandwich ELISA was carried out by coating 80027-1-RR at 80 ng/well followed by blocking and adding different concentration of eukaryotic expressed N protein (0.5-1000 ng/mL). HRP-conjugated80026-1-RR was used at 1 μg/mL for detection. Signal was developed with TMB and stopped by H2SO4 . Signal strength was measured by absorbance at 450 nm.

• 

  Affinity and Kinetic Characterization of 80027-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80027-1-RR against SARS-CoV-2 Nucleocapsid Phosphoprotein were performed. The affinity constant is 1.02 nM.

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Tested Applications

• Positive WB detected in: Eukaryotic nucleocapsid phosphoprotein, Ag30676
• Positive ELISA detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

80027-1-RR targets SARS-CoV-2 Nucleocapsid Phosphoprotein in WB, ELISA applications and shows reactivity with virus samples.

• Tested Reactivity: virus
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SARS-CoV-2 Nucleocapsid Phosphoprotein fusion protein Ag30676
• Full Name: COVID-19 N Protein
• GenBank Accession Number: NC_045512
• Gene Symbol: COVID-19 N Protein
• Gene ID (NCBI): 43740575
• RRID: AB_2882945
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity (PMID: 32416961, PMID: 32235387). A sandwich ELISA for COVID-19 N Protein can be assembled by using 80027-1-RR as capture antibody and conjugated 80026-1-RR for detection.