SARS-CoV-2 Nucleocapsid Phosphoprotein Polyclonal antibody

SARS-CoV-2 Nucleocapsid Phosphoprotein Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Virus and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 28769-1-AP

Synonyms

N protein, nucleocapsid phosphoprotein

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Validation Data Gallery

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  WB analysis using 28769-1-AP

  SARS-CoV-2 Nucleocapsid Phosphoprotein with the domain 1-419aa expressed in HEK-293 cells were subjected to SDS PAGE followed by western blot with 28769-1-AP (2019-nCOV nucleocapsid phosphoprotein antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  ELISA experiment of Recombinant protein using 28769-1-AP

  SARS-CoV-2 Nucleocapsid Phosphoprotein Antibody (28769-1-AP) tested by ELISA.SARS-CoV-2 Nucleocapsid Phosphoprotein was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Nucleocapsid Phosphoprotein Antibody (28769-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

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Tested Applications

• Positive WB detected in: Recombinant protein
• Positive ELISA detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

28769-1-AP targets SARS-CoV-2 Nucleocapsid Phosphoprotein in WB, ELISA applications and shows reactivity with Virus samples.

• Tested Reactivity: Virus
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SARS-CoV-2 Nucleocapsid Phosphoprotein fusion protein Ag30676
• Full Name: COVID-19 N Protein
• GenBank Accession Number: NC_045512
• Gene Symbol: COVID-19 N Protein
• Gene ID (NCBI): 43740575
• RRID: AB_2881212
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity.(PMID: 32416961, PMID: 32235387)

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; SARS-CoV-2 NSP5 and N protein counteract the RIG-I signaling pathway by suppressing the formation of stress granules.; Authors - Yi Zheng
human	WB	Antiviral Res; RBM24 inhibits the translation of SARS-CoV-2 polyproteins by targeting the 5ʹ-untranslated region; Authors - Yongxuan Yao
human	WB	Virol J; LINC01002 functions as a ceRNA to regulate FRMD8 by sponging miR-4324 for the development of COVID-19; Authors - Xinyi Kong