SARS-CoV-2 Nucleocapsid Phosphoprotein Polyclonal antibody

SARS-CoV-2 Nucleocapsid Phosphoprotein Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Virus and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28769-1-AP

Synonyms

N protein, nucleocapsid phosphoprotein



Tested Applications

Positive WB detected inRecombinant protein
Positive ELISA detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

28769-1-AP targets SARS-CoV-2 Nucleocapsid Phosphoprotein in WB, ELISA applications and shows reactivity with Virus samples.

Tested Reactivity Virus
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SARS-CoV-2 Nucleocapsid Phosphoprotein fusion protein Ag30676
Full Name COVID-19 N Protein
GenBank Accession NumberNC_045512
Gene Symbol COVID-19 N Protein
Gene ID (NCBI) 43740575
RRIDAB_2881212
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity.(PMID: 32416961, PMID: 32235387)

Protocols

Product Specific Protocols
WB protocol for SARS-CoV-2 Nucleocapsid Phosphoprotein antibody 28769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

SARS-CoV-2 NSP5 and N protein counteract the RIG-I signaling pathway by suppressing the formation of stress granules.

Authors - Yi Zheng
humanWB

Antiviral Res

RBM24 inhibits the translation of SARS-CoV-2 polyproteins by targeting the 5ʹ-untranslated region

Authors - Yongxuan Yao
humanWB

Virol J

LINC01002 functions as a ceRNA to regulate FRMD8 by sponging miR-4324 for the development of COVID-19

Authors - Xinyi Kong