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SARS-CoV-2 Membrane Glycoprotein Polyclonal antibody

SARS-CoV-2 Membrane Glycoprotein Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

virus

Applications

ELISA

Conjugate

Unconjugated

Cat no : 28882-1-AP

Synonyms

2019-nCOV membrane glycoprotein, M protein, membrane glycoprotein

Validation Data Gallery

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  • ELISA experiment of Recombinant protein using SARS-CoV-2 Membrane Glycoprotein Polyclonal antibo (28882-1-AP)

    ELISA experiment of Recombinant protein using 28882-1-AP

    COVID-19 M Protein Antibody (28882-1-AP) tested by ELISA. COVID-19 M Protein(101-222aa ) were coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of COVID-19 M Protein Antibody (28882-1-AP). Bound antibodies were detected with HRP conjugated Goat anti-Rabbit IgG followed by incubation with HRP Substrate and teminated with H2SO4, then measuring the resulting absorbance at 450 nm.

Tested Applications

Positive ELISA detected inRecombinant protein

Recommended dilution

ApplicationDilution
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28882-1-AP targets SARS-CoV-2 Membrane Glycoprotein in ELISA applications and shows reactivity with virus samples.

Tested Reactivity virus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SARS-CoV-2 Membrane Glycoprotein fusion protein Ag30691
Full Name COVID-19 M Protein
GenBank Accession NumberNC_045512
Gene Symbol SARS-CoV-2 Membrane Protein
Gene ID (NCBI) 43740571
RRIDAB_2881226
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide, 50% glycerol pH 7.3 and 0.05% BSA
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

In the coronaviruses, the membrane (M) protein was reported to be the most abundant viral protein expressed during infection and a key protein in the assembly of both naked and enveloped virus particles (PMID: 11967315). The M glycoprotein is conserved across the β-coronaviruses. The multiple sequence alignment shows a remarkable similarity (98% identity) among the Sars-CoV-2 M variants and the sequences from Bat and Pangolin isolates (PMID:32596311). The M protein is predicted to contain a triple-spanning transmembrane (TM) region, a single N-glycosylation site near its N-terminus that is in the exterior of the virion, and a long C-terminal region in the interior (PMID: 15626342).

Publications

SpeciesApplicationTitle

J Virol

Palmitoylation of SARS-CoV-2 Envelope protein is central to virus particle formation

Authors - Zhaohuan Wang
View Article