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Rat CD4 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: rat

Detection: rat

Applications

Sandwich ELISA

Range

Sandwich ELISA: 39.1-2500 pg/mL

Cat No : MP01387-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01387-3, Rat CD4 Recombinant Matched Antibody Pair - PBS only. 84530-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2025. 84530-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

    Sandwich ELISA standard curve of MP01387-3

    Sandwich ELISA standard curve of MP01387-3, Rat CD4 Recombinant Matched Antibody Pair - PBS only. 84530-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2025. 84530-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • The mean CD4 concentration was determined to be 874.9 pg/mL in C6 cell extract based on a 2.0 mg/mL extract load and 20,690.7 pg/mL in rat liver cell extract based on a 2.9 mg/mL extract load.

    Sample test of MP01387-3

    The mean CD4 concentration was determined to be 874.9 pg/mL in C6 cell extract based on a 2.0 mg/mL extract load and 20,690.7 pg/mL in rat liver cell extract based on a 2.9 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP01387-3 targets CD4 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 39.1-2500 pg/mL (Sandwich ELISA);

Capture antibody: Rat CD4 Recombinant antibody, PBS Only (Capture) 84530-4-PBS (241955F6). 100 μg. Concentration 1 mgl/ml.

Detection antibody: Rat CD4 Recombinant antibody, PBS Only (Detector) 84530-1-PBS (241955C8). 100 μg. Concentration 1 mgl/ml.

Alternative CD4 matched antibody pairs: MP01387-1, MP01387-2

Matched pair protein standard: Eg2025

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84530-4-PBS
Tested Reactivity rat
Immunogen Recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Cd4 molecule
Synonyms
GenBank accession numberNM_012705.1
Gene symbol Cd4
UniprotP05540
Gene ID (NCBI) 24932
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 84530-1-PBS
Tested Reactivity rat
Immunogen Recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Cd4 molecule
Synonyms p55, CD4 molecule, 241955C8
GenBank accession numberNM_012705.1
Gene symbol Cd4
UniprotP05540
Gene ID (NCBI) 24932
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.