Mouse Hif1a Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: Mouse

Detection: Mouse

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.156-10 ng/mL

Cat No : MP00056-2

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Synonyms

AA959795, bHLHe78, Hif1a, HIF1alpha, MOP1



Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00056-2 targets Hif1a in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.156-10 ng/mL (Sandwich ELISA);

Capture antibody: Mouse Hif1a Recombinant antibody, PBS Only (Capture) 82989-1-PBS (230101A3). 100 μg. Concentration 1 mgl/ml.

Detection antibody: Mouse Hif1a Recombinant antibody, PBS Only (Capture/Detector) 82989-3-PBS (230101H5). 100 μg. Concentration 1 mgl/ml.

Alternative Hif1a matched antibody pairs: MP00056-1

Matched pair protein standard: Ag33620

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 82989-1-PBS
Tested Reactivity Mouse
Immunogen Hif1a fusion protein Ag33620
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name hypoxia inducible factor 1, alpha subunit
Synonyms AA959795, bHLHe78, Hif1a, HIF1alpha, MOP1
GenBank accession numberNM_001313919
Gene symbol Hif1a
UniprotQ61221
Gene ID (NCBI) 15251
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 82989-3-PBS
Tested Reactivity Mouse
Immunogen Hif1a fusion protein Ag33620
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name hypoxia inducible factor 1, alpha subunit
Synonyms AA959795, bHLHe78, Hif1a, HIF1alpha, MOP1
GenBank accession numberNM_001313919
Gene symbol Hif1a
UniprotQ61221
Gene ID (NCBI) 15251
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.