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CoraLite®594-conjugated FKBP8 Monoclonal antibody

FKBP8 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C1G12

Cat no : CL594-66690

Synonyms

FKBP-38, FKBP38, FKBP 8, FKBP 38, FK506-binding protein 8

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite®594-conjugated FKBP8 Monoclonal antibody (CL594-66690)

    IF Staining of A431 using CL594-66690

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite®594 FKBP8 antibody (CL594-66690, Clone: 1C1G12 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of A431 cells using CoraLite®594-conjugated FKBP8 Monoclonal antibody (CL594-66690)

    FC experiment of A431 using CL594-66690

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human FKBP8 (CL594-66690, Clone:1C1G12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66690 targets FKBP8 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FKBP8 fusion protein Ag1649
Full Name FK506 binding protein 8, 38kDa
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC009966
Gene Symbol FKBP8
Gene ID (NCBI) 23770
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FK506 binding protein 8 (FKBP8), is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP8 does not seem to have PPIase/rotamase activity, which is different from other members of this family. The active form of FKBP8 may play a role in the regulation of apoptosis. FKBP8 has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for CL594 FKBP8 antibody CL594-66690Download protocol
FC protocol for CL594 FKBP8 antibody CL594-66690Download protocol
Standard Protocols
Click here to view our Standard Protocols