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Anti-Mouse CD5 Rabbit Recombinant Antibody

CD5 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240244G7

Cat no : 98038-1-RR

Synonyms

Lyt 1, Ly A, Ly 12, Ly 1, CD5 antigen

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse CD5 Rabbit Recombinant Antibody (98038-1-RR)

    FC experiment of mouse splenocytes using 98038-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD5 Rabbit Recombinant Antibody (98038-1-RR, Clone: 240244G7) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD3ε (145-2C11) (CL647-65060, Clone: 145-2C11). Cells were not fixed.

  • Flow cytometry (FC) experiment of mouse splenocytes using Anti-Mouse CD5 Rabbit Recombinant Antibody (98038-1-RR)

    FC experiment of mouse splenocytes using 98038-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD5 Rabbit Recombinant Antibody (98038-1-RR, Clone: 240244G7) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98038-1-RR

    Affinity and Kinetic Characterization of 98038-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98038-1-RR against Mouse CD5 were performed. The affinity constant is 0.701 nM.

Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98038-1-RR is CD5 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD5 antigen
Calculated Molecular Weight54 kDa
GenBank Accession NumberNM_007650.3
Gene Symbol Cd5
Gene ID (NCBI) 12507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

Protocols

Product Specific Protocols
FC protocol for CD5 antibody 98038-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols