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Anti-Human CD141/Thrombomodulin Rabbit Recombinant Antibody

CD141/Thrombomodulin Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240365E11

Cat no : 98037-1-RR

Synonyms

THBD, Thrombomodulin, THRM, Fetomodulin, CD141

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocyte using Anti-Human CD141/Thrombomodulin Rabbit Recombinant (98037-1-RR)

    FC experiment of human peripheral blood leukocyte using 98037-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD141/Thrombomodulin Rabbit Recombinant Antibody (98037-1-RR, Clone: 240365E11) or Isotype Control and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining.Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood leukocyte using Anti-Human CD141/Thrombomodulin Rabbit Recombinant (98037-1-RR)

    FC experiment of human peripheral blood leukocyte using 98037-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human Thrombomodulin Rabbit Recombinant Antibody (98037-1-RR, Clone: 240365E11) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98037-1-RR

    Affinity and Kinetic Characterization of 98037-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98037-1-RR against Human CD141/Thrombomodulin were performed. The affinity constant is 1.83 nM.

Tested Applications

Positive FC detected inhuman peripheral blood leukocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98037-1-RR is CD141/Thrombomodulin Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name thrombomodulin
Calculated Molecular Weight60 kDa
GenBank Accession NumberNM_000361.3
Gene Symbol THBD
Gene ID (NCBI) 7056
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood (PMID: 2827310). Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation (PMID: 9814688). In humans, thrombomodulin is encoded by the THBD gene. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. Thrombomodulin is glycosylated and has an apparent molecular weight of 75 to 110 kDa (PMID: 1650405; 2827310).

Protocols

Product Specific Protocols
FC protocol for CD141/Thrombomodulin antibody 98037-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols