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C12orf29 Recombinant antibody

C12orf29 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242163C3

Cat no : 84708-1-RR

Synonyms

Rnl, RNA Ligase 1, RNA Ligase, 242163C3

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using C12orf29 Recombinant antibody (84708-1-RR)

    WB analysis of HeLa using 84708-1-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 84708-1-RR (C12orf29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84708-1-RR

    Affinity and Kinetic Characterization of 84708-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84708-1-RR against Human C12orf29 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, U-87 MG cells, LNCaP cells, K-562 cells, rat pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84708-1-RR targets C12orf29 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen C12orf29 fusion protein Ag35681
Full Name chromosome 12 open reading frame 29
Calculated Molecular Weight37kDa，325aa
Observed Molecular Weight37 kDa
GenBank Accession NumberNM_001009894.2
Gene Symbol C12orf29
Gene ID (NCBI) 91298
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C12orf29, also known as RLIG1, is a 37 kDa human protein consisting of 325 amino acids. It catalyzes ATP-dependent RNA ligation via a three-step mechanism involving tandem auto- and RNA AMPylation (PMID: 36792600).

Protocols

Product Specific Protocols
WB protocol for C12orf29 antibody 84708-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols