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EPCAM/CD326 Recombinant antibody

EPCAM/CD326 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241851E11

Cat no : 84490-2-RR

Synonyms

Epithelial cell adhesion molecule, Ep-CAM, Epcam, EGP314, CD326

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPCAM/CD326 Recombinant antibody (84490-2-RR)

    WB analysis using 84490-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84490-2-RR (EpCAM/TROP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using EPCAM/CD326 Recombinant antibody (84490-2-RR)

    IHC staining of mouse colon using 84490-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84490-2-RR (EPCAM/CD326 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using EPCAM/CD326 Recombinant antibody (84490-2-RR)

    IHC staining of mouse colon using 84490-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84490-2-RR (EPCAM/CD326 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using EPCAM/CD326 Recombinant antibody (84490-2-RR)

    IHC staining of mouse small intestine using 84490-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 84490-2-RR (EPCAM/CD326 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using EPCAM/CD326 Recombinant antibody (84490-2-RR)

    IHC staining of mouse small intestine using 84490-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 84490-2-RR (EPCAM/CD326 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84490-2-RR

    Affinity and Kinetic Characterization of 84490-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84490-2-RR against Mouse EPCAM/CD326 were performed. The affinity constant is 0.779 nM.

Tested Applications

Positive WB detected inmouse colon tissue, mouse small intestine tissue
Positive IHC detected inmouse colon tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84490-2-RR targets EPCAM/CD326 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name epithelial cell adhesion molecule
Calculated Molecular Weight35 kDa
Observed Molecular Weight35 kDa, 40 kDa
GenBank Accession NumberNM_008532.2
Gene Symbol Epcam
Gene ID (NCBI) 17075
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

Protocols

Product Specific Protocols
WB protocol for EPCAM/CD326 antibody 84490-2-RRDownload protocol
IHC protocol for EPCAM/CD326 antibody 84490-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols