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Noggin Recombinant antibody

Noggin Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241511D10

Cat no : 84283-5-RR

Synonyms

NOG, SYNS1, SYM1, 241511D10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Noggin Recombinant antibody (84283-5-RR)

    WB analysis using 84283-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84283-5-RR (NOG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84283-5-RR

    Affinity and Kinetic Characterization of 84283-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84283-5-RR against Human Noggin were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inPC-3 cells, mouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84283-5-RR is Noggin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Noggin fusion protein Ag32755
Full Name noggin
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC034027
Gene Symbol Noggin
Gene ID (NCBI) 9241
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Noggin is an extracellular polypeptide acting as an antagonist of bone morphogenetic proteins (BMPs) regulating embryonal development. Noggin inhibits activity of BMP-2, -4, -7, -13, and -14. Noggin is present extracellularly in the matrix or retained at the cell surface via interaction with heparin sulfate proteoglycans. In early development stages, Noggin is produced by the Spemann organizer, allowing dorsal-ventral patterning of BMPs (PMID: 8752214). Subsequently, Noggin is expressed by the notochord regulating BMP-4 signaling in neurogenesis. Additionally, Noggin is present during development in the dermal papilla, connective tissue of the hair follicle, lens, retina, and periocular mesenchyme, as well as in the mesoderm lineage regulating development of the bone, cartilage, and muscles.

Protocols

Product Specific Protocols
WB protocol for Noggin antibody 84283-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols