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CD134/OX40 Recombinant antibody

CD134/OX40 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241398B4

Cat no : 84247-4-RR

Synonyms

TNFRSF4, OX40L receptor, OX40, CD134, ACT35 antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD134/OX40 Recombinant antibody (84247-4-RR)

    WB analysis using 84247-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84247-4-RR (OX40/TNFRSF4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84247-4-RR

    Affinity and Kinetic Characterization of 84247-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84247-4-RR against Human CD134/OX40 were performed. The affinity constant is 5.21 nM.

Tested Applications

Positive WB detected inMJ cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84247-4-RR is CD134/OX40 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name tumor necrosis factor receptor superfamily, member 4
Calculated Molecular Weight 29 kDa
Observed Molecular Weight45-50 kDa
GenBank Accession NumberNM_003327
Gene Symbol CD134
Gene ID (NCBI) 7293
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD134 (OX40) is a member of the TNFR-superfamily of receptors. Predominantly expressed on activated T cells, CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule. CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmunity (PMID: 26215166). CD134 can interact with TRAF2, TRAF3, and TRAF5.

Protocols

Product Specific Protocols
WB protocol for CD134/OX40 antibody 84247-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols