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OX40/TNFRSF4 Recombinant antibody, PBS Only (Capture/Detector)

OX40/TNFRSF4 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241398C10

Cat no : 84247-1-PBS

Synonyms

TNFRSF4, OX40L receptor, OX40, CD134, ACT35 antigen

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP01138-2

    Sandwich ELISA standard curve of MP01138-2

    Sandwich ELISA standard curve of MP01138-2, Human CD134/OX40 Recombinant Matched Antibody Pair - PBS only. 84247-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1261. 84247-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  • Sample test of MP01138-2

    Sample test of MP01138-2

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean CD134/OX40 concentration was not determined in unstimulated PBMC supernatant, 232.1 pg/mL in PHA stimulated PBMC supernatant.

  • Sample test of MP01138-2

    Sample test of MP01138-2

    The mean CD134/OX40 concentration was determined to be 4.0 ng/mL in Jurkat cell extract based on a 1.5 mg/mL extract load.

  • Sample test of MP01138-2

    Sample test of MP01138-2

    Serum of six individual healthy human donors was measured. The CD134/OX40 concentration of detected samples was determined to be 780.8 pg/mL with a range of 152.2-1,428.1 pg/mL.

  • Cytometric bead array standard curve of MP01138-2

    Cytometric bead array standard curve of MP01138-2

    Cytometric bead array standard curve of MP01138-2, CD134/OX40 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84247-2-PBS. Detection antibody: 84247-1-PBS. Standard: Eg1261. Range: 0.313-40 ng/mL.

  • Cytometric bead array standard curve of MP01138-3

    Cytometric bead array standard curve of MP01138-3

    Cytometric bead array standard curve of MP01138-3, CD134/OX40 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84247-1-PBS. Detection antibody: 84247-3-PBS. Standard: Eg1261. Range: 0.313-40 ng/mL.

Product Information

84247-1-PBS targets OX40/TNFRSF4 as part of a matched antibody pair:

MP01138-2: 84247-2-PBS capture and 84247-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01138-3: 84247-1-PBS capture and 84247-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein
Full Name tumor necrosis factor receptor superfamily, member 4
Calculated Molecular Weight 29 kDa
GenBank Accession NumberNM_003327
Gene Symbol CD134
Gene ID (NCBI) 7293
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.