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SRP19 Recombinant antibody

SRP19 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241219E9

Cat no : 84061-3-RR

Synonyms

241219E9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SRP19 Recombinant antibody (84061-3-RR)

    WB analysis using 84061-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84061-3-RR (SRP19 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SRP19 Recombinant antibody (84061-3-RR)

    IF Staining of HeLa using 84061-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SRP19 antibody (84061-3-RR, Clone: 241219E9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84061-3-RR

    Affinity and Kinetic Characterization of 84061-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84061-3-RR against Human SRP19 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, A549 cells, K-562 cells, Raji cells, mouse kidney tissue, mouse ovary tissue, mouse liver tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84061-3-RR is SRP19 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SRP19 fusion protein Ag8903
Full Name signal recognition particle 19kDa
Calculated Molecular Weight 144 aa, 16 kDa
Observed Molecular Weight17 kDa
GenBank Accession NumberBC010947
Gene Symbol SRP19
Gene ID (NCBI) 6728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The signal recognition particle (SRP) is one of the few functional small RNP particles. The SRP couples the synthesis of membrane and secretory proteins across or into the endoplasmic reticulum (ER) membrane in eukaryotes, as well as across the bacterial plasma membrane, and chloroplast thylakoid membranes. The mammalian SRP is composed of a 7S (or 7SL) RNA and six different proteins, SRP9, SRP14, SRP19, SRP54, SRP68 and SRP72. All of the components of SRP, including SRP RNA, participate directly in the overall protein targeting process. SRP19 binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP. SRP19 was shown to significantly enhance SRP54 attachment to helix 8 of 7SL RNA.

Protocols

Product Specific Protocols
WB protocol for SRP19 antibody 84061-3-RRDownload protocol
IF protocol for SRP19 antibody 84061-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols