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SRP54 Recombinant antibody

SRP54 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241125G1

Cat no : 84014-6-RR

Synonyms

Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4, 241125G1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SRP54 Recombinant antibody (84014-6-RR)

    WB analysis using 84014-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84014-6-RR (SRP54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SRP54 Recombinant antibody (84014-6-RR)

    WB analysis using 84014-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84014-6-RR (SRP54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SRP54 Recombinant antibody (84014-6-RR)

    IHC staining of human placenta using 84014-6-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84014-6-RR (SRP54 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84014-6-RR

    Affinity and Kinetic Characterization of 84014-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84014-6-RR against Human SRP54 were performed. The affinity constant is 4.24 nM.

Tested Applications

Positive WB detected inHeLa cells, RAW 264.7 cells, HEK-293 cells, MCF-7 cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84014-6-RR is SRP54 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SRP54 fusion protein Ag2327
Full Name signal recognition particle 54kDa
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC003389
Gene Symbol SRP54
Gene ID (NCBI) 6729
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).

Protocols

Product Specific Protocols
WB protocol for SRP54 antibody 84014-6-RRDownload protocol
IHC protocol for SRP54 antibody 84014-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols