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XBP1S-specific Recombinant antibody

XBP1S-specific Uni-rAbTM Recombinant Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241004C10

Cat no : 83959-6-RR

Synonyms

XBP1, X-box-binding protein 1, cytoplasmic form, X-box-binding protein 1, X box binding protein 1, TREB-5

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse liver tissue using XBP1S-specific Recombinant antibody (83959-6-RR)

    IHC staining of mouse liver using 83959-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83959-6-RR (XBP1S-specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using XBP1S-specific Recombinant antibody (83959-6-RR)

    IHC staining of mouse liver using 83959-6-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83959-6-RR (XBP1S-specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83959-6-RR

    Affinity and Kinetic Characterization of 83959-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83959-6-RR against Human XBP1S-specific were performed. The affinity constant is below 1 pM.

Tested Applications

Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83959-6-RR is XBP1S-specific Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen XBP1S-specific fusion protein Ag21703
Full Name X-box binding protein 1
Calculated Molecular Weight 261 aa, 29 kDa
GenBank Accession NumberBC000938
Gene Symbol XBP1
Gene ID (NCBI) 7494
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

XBP1, also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation (PMID: 14559994). XBP1 localizes in the nucleus. As a transcription factor, it is essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response. XBP1 has an association with major affective disorder. The unspliced XBP1U isoform is composed of 261 amino acid residues, and the spliced XBP1S isoform is composed of 376 amino acid residues.

Protocols

Product Specific Protocols
IHC protocol for XBP1S-specific antibody 83959-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols