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CEBPB Recombinant antibody

CEBPB Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240836G6

Cat no : 83791-6-RR

Synonyms

CCAAT/enhancer-binding protein beta, C/EBPbeta, C/EBP related protein 2, C/EBP beta, C EBP beta

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CEBPB Recombinant antibody (83791-6-RR)

    WB analysis using 83791-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83791-6-RR (CEBPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using CEBPB Recombinant antibody (83791-6-RR)

    IHC staining of human ovarian cancer using 83791-6-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83791-6-RR (CEBPB antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CEBPB Recombinant antibody (83791-6-RR)

    IF Staining of HepG2 using 83791-6-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEBPB antibody (83791-6-RR, Clone: 240836G6 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CEBPB Recombinant antibody (83791-6-RR)

    FC experiment of HeLa using 83791-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CEBPB Recombinant antibody (83791-6-RR, Clone:240836G6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83791-6-RR

    Affinity and Kinetic Characterization of 83791-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83791-6-RR against Human CEBPB were performed. The affinity constant is 69.2 pM.

Tested Applications

Positive WB detected inHeLa cells, A549 cells, U-87 MG cells, MKN-45 cells, MDA-MB-231 cells
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83791-6-RR is CEBPB Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CEBPB fusion protein Ag20073
Full Name CCAAT/enhancer binding protein (C/EBP), beta
Calculated Molecular Weight 345 aa, 36 kDa
Observed Molecular Weight42 kDa, 46 kDa
GenBank Accession NumberBC007538
Gene Symbol CEBPB
Gene ID (NCBI) 1051
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCAAT/enhancer-binding protein beta (CEBPB), also known as LAP, is a important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. It specifically binds to an IL-1 response element in the IL-6 gene. CEBPb mRNAs possess alternative translation-initiation codons, which result in the formation of truncated forms of the protein.Three variants of CEBPBs have been detected: a 46 kDa full-length liver-enriched transcription-activating protein (LAP1), a 42 kDa LAP2 and a 20 kDa liver-enriched transcription-inhibitory protein (LIP). (PMID:18820298).

Protocols

Product Specific Protocols
WB protocol for CEBPB antibody 83791-6-RRDownload protocol
IHC protocol for CEBPB antibody 83791-6-RRDownload protocol
IF protocol for CEBPB antibody 83791-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols