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RAP1A Recombinant antibody

RAP1A Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240438E10

Cat no : 83708-2-RR

Synonyms

Ras-related protein Krev-1, KREV1, GTP-binding protein smg p21A, G-22K, EC:3.6.5.2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAP1A Recombinant antibody (83708-2-RR)

    WB analysis using 83708-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83708-2-RR (RAP1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using RAP1A Recombinant antibody (83708-2-RR)

    FC experiment of HeLa using 83708-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAP1A Recombinant antibody (83708-2-RR, Clone:240438E10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83708-2-RR

    Affinity and Kinetic Characterization of 83708-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83708-2-RR against Human RAP1A were performed. The affinity constant is 0.232 nM.

Tested Applications

Positive WB detected inNIH/3T3 cells, A431 cells, HeLa cells, K-562 cells, human platelet tissue, rat brain tissue
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83708-2-RR is RAP1A Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RAP1A fusion protein Ag9488
Full Name RAP1A, member of RAS oncogene family
Calculated Molecular Weight 184 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC014086
Gene Symbol RAP1A
Gene ID (NCBI) 5906
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAP1A(Ras-related protein Rap-1A), also known as KREV1. Induces morphological reversion of a cell line transformed by a Ras oncogene. The biological roles of RAP1A include inducing neurite outgrowth and embryonic angiogenesis(PMID: 17916086). As one member of RAS oncogene family, it is a widely expressed receptor,with high mRNA expression in many tissues, particularly abundant in the colon. In cells, RAP1A was localized in cytoplasm and cell membrane(PMID: 11359771, PMID: 11359771).

Protocols

Product Specific Protocols
WB protocol for RAP1A antibody 83708-2-RRDownload protocol
FC protocol for RAP1A antibody 83708-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols