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METTL9 Recombinant antibody

METTL9 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240510A2

Cat no : 83531-5-RR

Synonyms

DREV1, DREV, CGI-81, CGI81, CGI 81

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METTL9 Recombinant antibody (83531-5-RR)

    WB analysis using 83531-5-RR

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 83531-5-RR (METTL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using METTL9 Recombinant antibody (83531-5-RR)

    FC experiment of HepG2 using 83531-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug METTL9 Recombinant antibody (83531-5-RR, Clone:240510A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83531-5-RR

    Affinity and Kinetic Characterization of 83531-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83531-5-RR against Human METTL9 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHEK-293 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83531-5-RR is METTL9 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen METTL9 fusion protein Ag7191
Full Name methyltransferase like 9
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC000195
Gene Symbol METTL9
Gene ID (NCBI) 51108
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

METTL9 is a broad-specificity methyltransferase that mediates the formation of the majority of 1-methylhistidine(1MH) present in mouse and human proteomes.

Protocols

Product Specific Protocols
WB protocol for METTL9 antibody 83531-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols