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CD5 Recombinant antibody

CD5 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240244C2

Cat no : 83451-4-RR

Synonyms

Lyt 1, Ly A, Ly 12, Ly 1, CD5 antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of EL-4 cells using CD5 Recombinant antibody (83451-4-RR)

    WB analysis of EL-4 using 83451-4-RR

    EL-4 cells were subjected to SDS PAGE followed by western blot with 83451-4-RR (CD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using CD5 Recombinant antibody (83451-4-RR)

    WB analysis of mouse thymus using 83451-4-RR

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 83451-4-RR (CD5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD5 Recombinant antibody (83451-4-RR)

    IHC staining of mouse spleen using 83451-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83451-4-RR (CD5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CD5 Recombinant antibody (83451-4-RR)

    IHC staining of mouse spleen using 83451-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83451-4-RR (CD5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83451-4-RR

    Affinity and Kinetic Characterization of 83451-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83451-4-RR against Mouse CD5 were performed. The affinity constant is 89.9 pM.

Tested Applications

Positive WB detected inEL-4 cells, mouse thymus tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83451-4-RR is CD5 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD5 antigen
Calculated Molecular Weight54 kDa
Observed Molecular Weight 55 kDa, 67 kDa
GenBank Accession NumberNM_007650.3
Gene Symbol Cd5
Gene ID (NCBI) 12507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

Protocols

Product Specific Protocols
WB protocol for CD5 antibody 83451-4-RRDownload protocol
IHC protocol for CD5 antibody 83451-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols