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Mouse VEGFR2/KDR Recombinant antibody, PBS Only (Capture)

VEGFR2/KDR Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240214D7

Cat no : 83366-1-PBS

Synonyms

VEGFR-2/Kdr, Kdr, Krd 1, Flk1, Flk 1

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using VEGFR2/KDR Recombinant antibody (83366-1-RR)

    WB analysis of mouse lung using 83366-1-RR (same clone as 83366-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 83366-1-RR (VEGFR2/KDR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83366-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00394-1

    Cytometric bead array standard curve of MP00394-1

    Cytometric bead array standard curve of MP00394-1, MOUSE VEGFR2/KDR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83366-1-PBS. Detection antibody: 83366-3-PBS. Standard: Eg0703. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00394-3

    Cytometric bead array standard curve of MP00394-3

    Cytometric bead array standard curve of MP00394-3, MOUSE VEGFR2/KDR Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83366-1-PBS. Detection antibody: 83366-2-PBS. Standard: Eg0703. Range: 5-80 ng/mL.

  • Affinity and Kinetic Characterization of 83366-1-RR

    Affinity and Kinetic Characterization of 83366-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83366-1-RR against Mouse VEGFR2/KDR were performed. The affinity constant is 1.88 nM.

Product Information

83366-1-PBS targets VEGFR2/KDR as part of a matched antibody pair:

MP00394-1: 83366-1-PBS capture and 83366-3-PBS detection (validated in Cytometric bead array)

MP00394-3: 83366-1-PBS capture and 83366-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name kinase insert domain protein receptor
Calculated Molecular Weight150KD
Observed Molecular Weight150-190 kDa
GenBank Accession NumberNM_010612.2
Gene Symbol VEGFR-2
Gene ID (NCBI) 16542
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VEGFR2, also named CD309, KDR, and FLK1, is a receptor for VEGF or VEGFC. VEGFR2 which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In the case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. VEGFR2 functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis, and sprouting.