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BAZ2A Recombinant antibody

BAZ2A Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230205H8

Cat no : 82910-1-RR

Synonyms

WALp3, TTF I interacting protein 5, TIP5, KIAA0314, hWALp3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BAZ2A Recombinant antibody (82910-1-RR)

    IF Staining of HepG2 using 82910-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BAZ2A antibody (82910-1-RR, Clone: 230205H8 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using BAZ2A Recombinant antibody (82910-1-RR)

    IF Staining of A549 using 82910-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using BAZ2A antibody (82910-1-RR, Clone: 230205H8 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green).

  • Affinity and Kinetic Characterization of 82910-1-RR

    Affinity and Kinetic Characterization of 82910-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82910-1-RR against Human BAZ2A were performed. The affinity constant is 1.46 nM.

Tested Applications

Positive IF/ICC detected inHepG2 cells, A549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82910-1-RR targets BAZ2A in IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen BAZ2A fusion protein Ag33963
Full Name bromodomain adjacent to zinc finger domain, 2A
Calculated Molecular Weight211 kDa
GenBank Accession NumberNM_013449.4
Gene Symbol BAZ2A
Gene ID (NCBI) 11176
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAZ2A (bromodomain adjacent to zinc finger domain 2A), also known as TIP5. It is expected to be located in nucleus, and the protein is expressed at moderate levels in most tissues analyzed, including heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. BAZ2A is an epigenetic regulator affecting transcription of ribosomal RNA, it is also a regulatory subunit of the ATP-dependent NoRC-1 and NoRC-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair (PubMed:28801535). The calculated molecular weight of BAZ2A is 210 kDa.

Protocols

Product Specific Protocols
IF protocol for BAZ2A antibody 82910-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols