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Phospho-EIF2AK4 (Thr899) Recombinant antibody

Phospho-EIF2AK4 (Thr899) Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

5N13

Cat no : 82835-3-RR

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-EIF2AK4 (Thr899) Recombinant antibody (82835-3-RR)

    WB analysis using 82835-3-RR

    Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 82835-3-RR (Phospho-EIF2AK4 (Thr899) antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin (66095-1-Ig) antibody as a loading control.

  • Western Blot (WB) analysis of various lysates using Phospho-EIF2AK4 (Thr899) Recombinant antibody (82835-3-RR)

    WB analysis using 82835-3-RR

    Non-treated and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82835-3-RR (Phospho-EIF2AK4 (Thr899) antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin (66095-1-Ig) antibody as a loading control.

Tested Applications

Positive WB detected inCalyculin A treated HeLa cells, Calyculin A treated NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82835-3-RR targets Phospho-EIF2AK4 (Thr899) in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name eukaryotic translation initiation factor 2 alpha kinase 4
Calculated Molecular Weight 187 kDa
Observed Molecular Weight187 kDa
GenBank Accession NumberBC009350
Gene Symbol EIF2AK4
Gene ID (NCBI) 440275
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EIF2AK4 (eukaryotic translation initiation factor 2 alpha kinase 4) belongs to a family of protein kinases that phosphorylate the α subunit of EIF2 (eukaryotic translation initiation factor 2) in response to various stress stimuli. Phosphorylation of EIF2A in response to EIF2AK4 activation subsequently increases translation of specific mRNAs that are important for stress remediation such as ATF4 (activating transcription factor 4). (PMID: 26986695)

Protocols

Product Specific Protocols
WB protocol for Phospho-EIF2AK4 (Thr899) antibody 82835-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols