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ICAM-1/CD54 Recombinant antibody

ICAM-1/CD54 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240249H6

Cat no : 82827-8-RR

Synonyms

CD54, Icam-1, MALA-2, Icam1, ICAM 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ICAM1 Recombinant antibody (82827-8-RR)

    WB analysis using 82827-8-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82827-8-RR (ICAM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82827-8-RR

    Affinity and Kinetic Characterization of 82827-8-RR

    Biolayer interferometry (BLl) kinetic assays of 82827-8-RR against Mouse ICAM-1 were performed. The affinity constant is 0.714 nM.

Tested Applications

Positive WB detected inmouse kidney tissue, mouse lung tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82827-8-RR is ICAM-1/CD54 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name intercellular adhesion molecule 1
Calculated Molecular Weight59 kDa
Observed Molecular Weight85-110 kDa
GenBank Accession NumberNM_010493
Gene Symbol ICAM-1
Gene ID (NCBI) 15894
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ICAM-1 (CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

Protocols

Product Specific Protocols
WB protocol for ICAM-1/CD54 antibody 82827-8-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols