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  • Featured Product
  • KD/KO Validated

HBS1L Recombinant antibody

HBS1L Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2L24

Cat no : 82795-2-RR

Synonyms

DKFZp686L13262, EF 1a, ERFS, HBS1, HBS1 like (S. cerevisiae), HBS1 like protein, HBS1L, HSPC276, KIAA1038

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HBS1L Recombinant antibody (82795-2-RR)

    WB analysis using 82795-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82795-2-RR (HBS1L antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using HBS1L Recombinant antibody (82795-2-RR)

    WB analysis of HEK-293 using 82795-2-RR

    WB result of HBS1L antibody (82795-2-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-HBS1L transfected HEK-293 cells.

  • Affinity and Kinetic Characterization of 82795-2-RR

    Affinity and Kinetic Characterization of 82795-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82795-2-RR against Human HBS1L were performed. The affinity constant is 0.931 nM.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82795-2-RR targets HBS1L in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HBS1L fusion protein Ag0388
Full Name HBS1-like (S. cerevisiae)
Calculated Molecular Weight 75 kDa
Observed Molecular Weight78 kDa
GenBank Accession NumberBC001465
Gene Symbol HBS1L
Gene ID (NCBI) 10767
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HBS1L (HBS1-like), also known as EF-1a or ERFS, is a 684 amino acid protein that belongs to the GTP-binding elongation factor family and exists as multiple alternatively spliced isoforms. Expressed in kidney, brain, heart, placenta, liver, muscle and pancreas, HSB1L is thought to play a role in controlling fetal hemoglobin levels, specifically influencing platelet, monocyte and erythrocyte hemoglobin content. The gene encoding HBS1L maps to a locus on human chromosome 6 that is associated with sickle cell anemia and beta-thalassemia, suggesting a role for HBS1L in the pathogenesis of blood disorders.

Protocols

Product Specific Protocols
WB protocol for HBS1L antibody 82795-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols