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CPEB1 Recombinant antibody

CPEB1 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

6I9

Cat no : 82785-1-RR

Synonyms

CPE-binding protein 1, CPEB, CPE BP1, CPE binding protein 1, CEBP

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CPEB1 Recombinant antibody (82785-1-RR)

    IF Staining of HeLa using 82785-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CPEB1 antibody (82785-1-RR, Clone: 6I9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • Affinity and Kinetic Characterization of 82785-1-RR

    Affinity and Kinetic Characterization of 82785-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82785-1-RR against Human CPEB1 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82785-1-RR targets CPEB1 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CPEB1 fusion protein Ag3994
Full Name cytoplasmic polyadenylation element binding protein 1
Calculated Molecular Weight 486 aa, 54 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC035348
Gene Symbol CPEB1
Gene ID (NCBI) 64506
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CPEB1, also named as CEBP, CPEB and CPE-BP1, contains two RRM (RNA recognition motif) domains and belongs to the RRM CPEB family. It is a sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. CPEB1 regulated translation is a key process involved in INS signaling.

Protocols

Product Specific Protocols
IF protocol for CPEB1 antibody 82785-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols