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EIF2B1 Monoclonal antibody

EIF2B1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H6A3

Cat no : 68824-1-Ig

Synonyms

EIF2BA, eIF2B GDP-GTP exchange factor subunit alpha, EIF2B, EIF 2Balpha, EIF 2B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF2B1 Monoclonal antibody (68824-1-Ig)

    WB analysis using 68824-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68824-1-Ig (EIF2B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using EIF2B1 Monoclonal antibody (68824-1-Ig)

    WB analysis of NIH/3T3 using 68824-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68824-1-Ig (EIF2B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EIF2B1 Monoclonal antibody (68824-1-Ig)

    IF Staining of HeLa using 68824-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF2B1 antibody (68824-1-Ig, Clone: 1H6A3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

Tested Applications

Positive WB detected inNIH/3T3 cells, U2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68824-1-Ig targets EIF2B1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EIF2B1 fusion protein Ag12493
Full Name eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa
Calculated Molecular Weight 305 aa, 34 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC103763
Gene Symbol EIF2B1
Gene ID (NCBI) 1967
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EIF2B1 antibody 68824-1-IgDownload protocol
IF protocol for EIF2B1 antibody 68824-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols