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IKBKE Monoclonal antibody

IKBKE Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3E4H7

Cat no : 68531-1-Ig

Synonyms

I kappa B kinase epsilon, IKBKE, IKK E, IKK epsilon, IKK i, IKKE, IKKI, Inducible I kappa B kinase, KIAA0151

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IKBKE Monoclonal antibody (68531-1-Ig)

    WB analysis using 68531-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68531-1-Ig (IKBKE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using IKBKE Monoclonal antibody (68531-1-Ig)

    WB analysis using 68531-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68531-1-Ig (IKBKE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inSK-BR-3 cells, Jurkat cells, MCF-7 cells, HeLa cells, Raji cells, Ramos cells, Daudi cells, HL-60 cells, THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68531-1-Ig targets IKBKE in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IKBKE fusion protein Ag30374
Full Name inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
Calculated Molecular Weight 716 aa, 80 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC107812
Gene Symbol IKBKE
Gene ID (NCBI) 9641
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IKBKE is a serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. It is also involved in TNFA and inflammatory cytokines, like Interleukin-1, signaling.

Protocols

Product Specific Protocols
WB protocol for IKBKE antibody 68531-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols