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HDDC2 Monoclonal antibody

HDDC2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2E1B2

Cat no : 68391-1-Ig

Synonyms

EC:3.1.3.89, CGI-130, CGI 130, C6orf74, 5'-deoxynucleotidase HDDC2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HDDC2 Monoclonal antibody (68391-1-Ig)

    WB analysis using 68391-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68391-1-Ig (HDDC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using HDDC2 Monoclonal antibody (68391-1-Ig)

    IHC staining of mouse kidney using 68391-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68391-1-Ig (HDDC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using HDDC2 Monoclonal antibody (68391-1-Ig)

    IHC staining of rat brain using 68391-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68391-1-Ig (HDDC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Saos-2 cells using HDDC2 Monoclonal antibody (68391-1-Ig)

    IF Staining of Saos-2 using 68391-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using HDDC2 antibody (68391-1-Ig, Clone: 2E1B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of U2OS cells using HDDC2 Monoclonal antibody (68391-1-Ig)

    FC experiment of U2OS using 68391-1-Ig

    1x10^6 U2OS cells were intracellularly stained with 0.4 ug HDDC2 Monoclonal antibody (68391-1-Ig, Clone:2E1B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inA549 cells, MCF-7 cells
Positive IHC detected inmouse kidney tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSaos-2 cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68391-1-Ig targets HDDC2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen HDDC2 fusion protein Ag33258
Full Name HD domain containing 2
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC003357
Gene Symbol HDDC2
Gene ID (NCBI) 51020
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HDDC2, also named as C6orf74, plays a role in the maintenance of pluripotency in human ES and iPS cells. HDDC2 is a nuclear‐encoded but mitochondrially localized protein. It has been reported as associated with oxidative metabolism.

Protocols

Product Specific Protocols
WB protocol for HDDC2 antibody 68391-1-IgDownload protocol
IHC protocol for HDDC2 antibody 68391-1-IgDownload protocol
IF protocol for HDDC2 antibody 68391-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols