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SGF29 Monoclonal antibody

SGF29 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G1B4

Cat no : 68339-1-Ig

Synonyms

CCDC101, FLJ32446, SGF29

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SGF29 Monoclonal antibody (68339-1-Ig)

    WB analysis using 68339-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68339-1-Ig (CCDC101 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

Tested Applications

Positive WB detected inLNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68339-1-Ig targets SGF29 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SGF29 fusion protein Ag33219
Full Name coiled-coil domain containing 101
Calculated Molecular Weight 293 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC011981
Gene Symbol SGF29
Gene ID (NCBI) 112869
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SAGA-associated factor 29 (SGF29) is a chromatin reader component of some histone acetyltransferase (HAT) SAGA-type complexes. SGF29 specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3) (PMID:19103755, PMID:26421618, PMID:21685874, PMID:26578293). Involved in the response to the endoplasmic reticulum (ER) stress by recruiting the SAGA complex to H3K4me, thereby promoting histone H3 acetylation and cell survival (PubMed:23894581). It has a calculated molecular weight of 33 kDa.

Protocols

Product Specific Protocols
WB protocol for SGF29 antibody 68339-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols