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KIAA2013 Monoclonal antibody

KIAA2013 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

4G6C4

Cat no : 68245-1-Ig

Synonyms

KIAA2013

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using KIAA2013 Monoclonal antibody (68245-1-Ig)

    WB analysis using 68245-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68245-1-Ig (KIAA2013 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using KIAA2013 Monoclonal antibody (68245-1-Ig)

    WB analysis using 68245-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68245-1-Ig (KIAA2013 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68245-1-Ig targets KIAA2013 in WB, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen KIAA2013 fusion protein Ag27438
Full Name KIAA2013
Observed Molecular Weight65 kDa
GenBank Accession NumberBC035033
Gene Symbol KIAA2013
Gene ID (NCBI) 90231
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIAA2013, also known as Q8IYS2 or MGC33867, is a single-pass transmembrane protein encoded by the KIAA2013 gene in humans. KIAA2013 has been found to play a role in the Endocannabinoid system. This system is made up of cannabinoid receptors 1 and 2 (CB1 and CB2) as well as the various ligands and enzymes that interact. The protein KIAA2013 has been found to be expressed within CB2 expressing cells. Both cannabinoid receptors are labeled as class A G-Protein Coupled Receptors, and CB2 is highly expressed within the human spleen and leukocytes. CB2, and by extension KIAA2013, are therefore targets of interest for therapeutic studies looking into diseases such as inflammatory bowel disease and rheumatoid arthritis(PMID:31616248 34752341).

Protocols

Product Specific Protocols
WB protocol for KIAA2013 antibody 68245-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols