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ARHGEF16 Monoclonal antibody

ARHGEF16 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D8E3

Cat no : 67975-1-Ig

Synonyms

ARHGEF16, GEF16, NBR

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARHGEF16 Monoclonal antibody (67975-1-Ig)

    WB analysis using 67975-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67975-1-Ig (ARHGEF16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ARHGEF16 Monoclonal antibody (67975-1-Ig)

    WB analysis of HepG2 using 67975-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67975-1-Ig (ARHGEF16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ARHGEF16 Monoclonal antibody (67975-1-Ig)

    WB analysis of MCF-7 using 67975-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67975-1-Ig (ARHGEF16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ARHGEF16 Monoclonal antibody (67975-1-Ig)

    IF Staining of MCF-7 using 67975-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ARHGEF16 antibody (67975-1-Ig, Clone: 1D8E3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, HepG2 cells, Hela cells, HEK-293 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67975-1-Ig targets ARHGEF16 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ARHGEF16 fusion protein Ag28675
Full Name Rho guanine exchange factor (GEF) 16
Calculated Molecular Weight 80 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC002681
Gene Symbol ARHGEF16
Gene ID (NCBI) 27237
RRIDAB_2918724
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Small GTPases of the Rho family are critical regulators of various cellular functions including actin cytoskeleton organization, activation of kinase cascades, mitogenesis, transcriptional activation and stimulation of DNA synthesis. Rho proteins cycle between a biologically active GTP-bound state and an inactive GDP-bound state. Rho guanine nucleotide exchange factor (GEF) has a crucial role in activating small GTPase by exchange GDP for GTP. ARGEF16 is one of the multiple GEF members. Although the specific function of this protein is not known yet, it is thought to be involved in protein-protein and protein-lipid interactions.

Protocols

Product Specific Protocols
WB protocol for ARHGEF16 antibody 67975-1-IgDownload protocol
IF protocol for ARHGEF16 antibody 67975-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols