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MGME1 Monoclonal antibody

MGME1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C4G1

Cat no : 67468-1-Ig

Synonyms

bA504H3.4, C20orf72, FLJ14597, MGME1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MGME1 Monoclonal antibody (67468-1-Ig)

    WB analysis using 67468-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67468-1-Ig (MGME1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MGME1 Monoclonal antibody (67468-1-Ig)

    WB analysis of HeLa using 67468-1-Ig

    WB result of MGME1 antibody (67468-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MGME1 transfected HeLa cells.

  • Western Blot (WB) analysis of HL-60 cells using MGME1 Monoclonal antibody (67468-1-Ig)

    WB analysis of HL-60 using 67468-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 67468-1-Ig (MGME1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MGME1 Monoclonal antibody (67468-1-Ig)

    IHC staining of human lung cancer using 67468-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using MGME1 Monoclonal antibody (67468-1-Ig)

    IHC staining of human lung cancer using 67468-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MGME1 Monoclonal antibody (67468-1-Ig)

    IHC staining of human colon cancer using 67468-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MGME1 Monoclonal antibody (67468-1-Ig)

    IHC staining of human colon cancer using 67468-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MGME1 Monoclonal antibody (67468-1-Ig)

    IHC staining of human colon cancer using 67468-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MGME1 Monoclonal antibody (67468-1-Ig)

    IHC staining of human colon cancer using 67468-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67468-1-Ig (MGME1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HL-60 cells, HepG2 cells, LNCaP cells, A549 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67468-1-Ig targets MGME1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MGME1 fusion protein Ag19644
Full Name chromosome 20 open reading frame 72
Calculated Molecular Weight 344 aa, 39 kDa
Observed Molecular Weight 36-39 kDa
GenBank Accession NumberBC016869
Gene Symbol MGME1
Gene ID (NCBI) 92667
RRIDAB_2882697
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MGME1 antibody 67468-1-IgDownload protocol
IHC protocol for MGME1 antibody 67468-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

MGME1 associates with poor prognosis and is vital for cell proliferation in lower-grade glioma

Authors - Feng Xiao
View Article