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RCC1 Monoclonal antibody

RCC1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C2F5

Cat no : 67335-1-Ig

Synonyms

Cell cycle regulatory protein, CHC1, RCC1, RCC1 I

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RCC1 Monoclonal antibody (67335-1-Ig)

    WB analysis using 67335-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67335-1-Ig (RCC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RCC1 Monoclonal antibody (67335-1-Ig)

    IHC staining of mouse brain using 67335-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67335-1-Ig (RCC1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RCC1 Monoclonal antibody (67335-1-Ig)

    IHC staining of human stomach cancer using 67335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67335-1-Ig (RCC1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RCC1 Monoclonal antibody (67335-1-Ig)

    IHC staining of human stomach cancer using 67335-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67335-1-Ig (RCC1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RCC1 Monoclonal antibody (67335-1-Ig)

    IHC staining of mouse brain using 67335-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67335-1-Ig (RCC1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, LNCaP cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inmouse brain tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67335-1-Ig targets RCC1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RCC1 fusion protein Ag17809
Full Name regulator of chromosome condensation 1
Calculated Molecular Weight 421 aa, 45 kDa
Observed Molecular Weight45-50 kDa
GenBank Accession NumberBC010067
Gene Symbol RCC1
Gene ID (NCBI) 1104
RRIDAB_2882593
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation.

Protocols

Product Specific Protocols
WB protocol for RCC1 antibody 67335-1-IgDownload protocol
IHC protocol for RCC1 antibody 67335-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols