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CD300A Monoclonal antibody, PBS Only (Detector)

CD300A Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F9C5

Cat no : 67242-1-PBS

Synonyms

CMRF 35 H9, CLM-8, CLM 8, CD300a molecule, CD300 antigen-like family member A

Validation Data Gallery

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  • Western Blot (WB) analysis of THP-1 cells using CD300A Monoclonal antibody (67242-1-Ig)

    WB analysis of THP-1 using 67242-1-Ig (same clone as 67242-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67242-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using CD300A Monoclonal antibody (67242-1-Ig)

    WB analysis of K-562 using 67242-1-Ig (same clone as 67242-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67242-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using CD300A Monoclonal antibody (67242-1-Ig)

    WB analysis of HL-60 using 67242-1-Ig (same clone as 67242-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67242-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human spleen tissue using CD300A Monoclonal antibody (67242-1-Ig)

    WB analysis of human spleen using 67242-1-Ig (same clone as 67242-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67242-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using CD300A Monoclonal antibody (67242-1-Ig)

    WB analysis of Raji using 67242-1-Ig (same clone as 67242-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67242-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CD300A Monoclonal antibody (67242-1-Ig)

    WB analysis of Jurkat using 67242-1-Ig (same clone as 67242-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67242-1-Ig (CD300A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67242-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50466-1

    Cytometric bead array standard curve of MP50466-1

    Cytometric bead array standard curve of MP50466-1, CD300A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67242-2-PBS. Detection antibody: 67242-1-PBS. Standard:Ag5776. Range: 0.391-100 ng/mL.

Product Information

67242-1-PBS targets CD300A as part of a matched antibody pair:

MP50466-1: 67242-2-PBS capture and 67242-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD300A fusion protein Ag5776
Full Name CD300a molecule
Calculated Molecular Weight 299 aa, 33 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC032352
Gene Symbol CD300A
Gene ID (NCBI) 11314
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.