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SIN3A Monoclonal antibody

SIN3A Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B12E8

Cat no : 67197-1-Ig

Synonyms

DKFZp434K2235, FLJ90319, KIAA0700, SIN3A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIN3A Monoclonal antibody (67197-1-Ig)

    WB analysis using 67197-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67197-1-Ig (SIN3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using SIN3A Monoclonal antibody (67197-1-Ig)

    WB analysis of HSC-T6 using 67197-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67197-1-Ig (SIN3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using SIN3A Monoclonal antibody (67197-1-Ig)

    WB analysis of NIH/3T3 using 67197-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67197-1-Ig (SIN3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of 4T1 cells using SIN3A Monoclonal antibody (67197-1-Ig)

    WB analysis of 4T1 using 67197-1-Ig

    4T1 cells were subjected to SDS PAGE followed by western blot with 67197-1-Ig (SIN3A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using SIN3A Monoclonal antibody (67197-1-Ig)

    IF Staining of U2OS using 67197-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SIN3A antibody (67197-1-Ig, Clone: 2B12E8 ) at dilution of 1:2500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

Tested Applications

Positive WB detected inHeLa cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, HEK-293 cells, MCF-7 cells, Jurkat cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:1250-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67197-1-Ig targets SIN3A in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SIN3A fusion protein Ag28865
Full Name SIN3 homolog A, transcription regulator (yeast)
Calculated Molecular Weight 145 kDa
Observed Molecular Weight 145-150 kDa
GenBank Accession NumberBC066364
Gene Symbol SIN3A
Gene ID (NCBI) 25942
RRIDAB_2882490
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SIN3A antibody 67197-1-IgDownload protocol
IF protocol for SIN3A antibody 67197-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols