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VEGFC Monoclonal antibody

VEGFC Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B11F7

Cat no : 67116-1-Ig

Synonyms

Flt4 L, Flt4 ligand, VEGF C, VEGFC, VRP

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using VEGFC Monoclonal antibody (67116-1-Ig)

    WB analysis of MCF-7 using 67116-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67116-1-Ig (VEGFC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of T-47D cells using VEGFC Monoclonal antibody (67116-1-Ig)

    WB analysis of T-47D using 67116-1-Ig

    T-47D cells were subjected to SDS PAGE followed by western blot with 67116-1-Ig (VEGFC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using VEGFC Monoclonal antibody (67116-1-Ig)

    WB analysis of LNCaP using 67116-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67116-1-Ig (VEGFC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCI-H1299 cells using VEGFC Monoclonal antibody (67116-1-Ig)

    WB analysis of NCI-H1299 using 67116-1-Ig

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67116-1-Ig (VEGFC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using VEGFC Monoclonal antibody (67116-1-Ig)

    WB analysis of HT-1080 using 67116-1-Ig

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 67116-1-Ig (VEGFC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of T-47D cells using VEGFC Monoclonal antibody (67116-1-Ig)

    WB analysis of T-47D using 67116-1-Ig

    T-47D cells were subjected to SDS PAGE followed by western blot with 67116-1-Ig (VEGFC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using VEGFC Monoclonal antibody (67116-1-Ig)

    WB analysis of A549 using 67116-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67116-1-Ig (VEGFC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using VEGFC Monoclonal antibody (67116-1-Ig)

    IF Staining of MCF-7 using 67116-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using VEGFC antibody (67116-1-Ig, Clone: 2B11F7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inMCF-7 cells, T-47D cells, NCI-H1299 cells, A549 cells, LNCaP cells, HT-1080 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67116-1-Ig targets VEGFC in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VEGFC fusion protein Ag18182
Full Name vascular endothelial growth factor C
Calculated Molecular Weight 419 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC035212
Gene Symbol VEGFC
Gene ID (NCBI) 7424
RRIDAB_2882420
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for VEGFC antibody 67116-1-IgDownload protocol
IF protocol for VEGFC antibody 67116-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Cycle

CircHGS enhances the progression of bladder cancer by regulating the miR-513a-5p/VEGFC axis and activating the AKT/mTOR signaling pathway

Authors - Yi Zhu
View Article