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AGXT2 Monoclonal antibody

AGXT2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse and More (2)

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

3A5B12

Cat no : 66602-1-Ig

Synonyms

AGT 2, AGT2, AGXT2, Beta ALAAT II, D AIBAT

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AGXT2 Monoclonal antibody (66602-1-Ig)

    WB analysis using 66602-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66602-1-Ig (AGXT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using AGXT2 Monoclonal antibody (66602-1-Ig)

    WB analysis of HeLa using 66602-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66602-1-Ig (AGXT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using AGXT2 Monoclonal antibody (66602-1-Ig)

    WB analysis of Jurkat using 66602-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66602-1-Ig (AGXT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AGXT2 Monoclonal antibody (66602-1-Ig)

    IHC staining of human kidney using 66602-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66602-1-Ig (AGXT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using AGXT2 Monoclonal antibody (66602-1-Ig)

    IHC staining of human kidney using 66602-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66602-1-Ig (AGXT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66602-1-Ig targets AGXT2 in WB, IHC, CoIP, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AGXT2 fusion protein Ag26888
Full Name alanine-glyoxylate aminotransferase 2
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberNM_001306173
Gene Symbol AGXT2
Gene ID (NCBI) 64902
RRIDAB_2881962
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AGXT2 antibody 66602-1-IgDownload protocol
IHC protocol for AGXT2 antibody 66602-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,CoIP

J Hepatocell Carcinoma

AGXT2 Suppresses the Proliferation and Dissemination of Hepatocellular Carcinoma Cells by Modulating Intracellular Lipid Metabolism

Authors - Tian Chen
  • KD Validated
View Article