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Anti-Human CD11b (ICRF44)

CD11b Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human and More (1)

Applications

FC

Conjugate

Unconjugated

CloneNo.

ICRF44

Cat no : 65116-1-Ig

Synonyms

CD11B, CR 3 alpha chain, CR3A, Integrin alpha M, ITGAM, MAC 1, MAC1A, MO1A, Neutrophil adherence receptor, SLEB6

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD11b (ICRF44) (65116-1-Ig)

    FC experiment of human PBMCs using 65116-1-Ig

    1X10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD11b (65116-1-Ig, Clone:ICRF44) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red) or unstained. Cells were not fixed. Monocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD11b (ICRF44) (65116-1-Ig)

    FC experiment of human PBMCs using 65116-1-Ig

    1X10^6 human PBMCs were surface stained with 0.5 ug Anti-Human CD11b (65116-1-Ig, Clone:ICRF44) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 2 publications below

Product Information

65116-1-Ig targets CD11b in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Rheumatoid synovial cells and human monocytes
Full Name integrin, alpha M (complement component 3 receptor 3 subunit)
Calculated Molecular Weight 1152 aa, 127 kDa
GenBank Accession NumberBC096346
Gene Symbol CD11b
Gene ID (NCBI) 3684
RRIDAB_2918412
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Protocols

Product Specific Protocols
FC protocol for CD11b antibody 65116-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

Front Cell Dev Biol

EZH2-Inhibited MicroRNA-454-3p Promotes M2 Macrophage Polarization in Glioma.

Authors - Bin Qi
View Article
humanFC

J Med Chem

Discovery of Novel, Potent, and Selective Small-Molecule Menin-Mixed Lineage Leukemia Interaction Inhibitors through Attempting Introduction of Hydrophilic Groups

Authors - Hao Lei
View Article