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C7orf27 Polyclonal antibody

C7orf27 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31921-1-AP

Synonyms

BRCA1-associated protein required for ATM activation protein 1, BRCA1-associated ATM activator 1, BRCA1 Associated ATM Activator 1, BRAT1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using C7orf27 Polyclonal antibody (31921-1-AP)

    WB analysis using 31921-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31921-1-AP (C7orf27 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using C7orf27 Polyclonal antibody (31921-1-AP)

    IHC staining of human placenta using 31921-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31921-1-AP (C7orf27 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using C7orf27 Polyclonal antibody (31921-1-AP)

    IF Staining of HEK-293 using 31921-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C7orf27 antibody (31921-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, Hela cells, Jurkat cells, U2OS cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31921-1-AP targets C7orf27 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C7orf27 fusion protein Ag37056
Full Name chromosome 7 open reading frame 27
Observed Molecular Weight75 kDa
GenBank Accession NumberBC015632
Gene Symbol C7orf27
Gene ID (NCBI) 221927
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C7orf27, also known as BRAT1, not only plays a role in DNA damage response, but also participates in the regulation of cell growth and apoptosis, which further regulates mitochondrial metabolism and tumorigenesis. Moreover, several studies have shown that BRAT1 mutations are associated with neurodevelopmental and neurodegenerative diseases.

Protocols

Product Specific Protocols
WB protocol for C7orf27 antibody 31921-1-APDownload protocol
IHC protocol for C7orf27 antibody 31921-1-APDownload protocol
IF protocol for C7orf27 antibody 31921-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols