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CD38 Polyclonal antibody

CD38 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31891-1-AP

Synonyms

ADP-ribosyl cyclase 1, ADPRC 1, ADP ribosyl cyclase 1, 2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD38 Polyclonal antibody (31891-1-AP)

    WB analysis using 31891-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31891-1-AP (CD38 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD38 Polyclonal antibody (31891-1-AP)

    IHC staining of human tonsillitis using 31891-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31891-1-AP (CD38 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD38 Polyclonal antibody (31891-1-AP)

    IHC staining of human tonsillitis using 31891-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 31891-1-AP (CD38 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inMOLT-4 cells, Raji cells, Ramos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31891-1-AP targets CD38 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein
Full Name CD38 molecule
Calculated Molecular Weight 300 aa, 34 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC007964
Gene Symbol CD38
Gene ID (NCBI) 952
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).

Protocols

Product Specific Protocols
WB protocol for CD38 antibody 31891-1-APDownload protocol
IHC protocol for CD38 antibody 31891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols