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PTPRS Polyclonal antibody

PTPRS Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 31720-1-AP

Synonyms

Receptor-type tyrosine-protein phosphatase sigma, Receptor-type tyrosine-protein phosphatase S, R PTP sigma, R PTP S, PTPSIGMA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPRS Polyclonal antibody (31720-1-AP)

    WB analysis using 31720-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31720-1-AP (PTPRS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PTPRS Polyclonal antibody (31720-1-AP)

    IHC staining of mouse cerebellum using 31720-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31720-1-AP (PTPRS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using PTPRS Polyclonal antibody (31720-1-AP)

    IHC staining of mouse cerebellum using 31720-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31720-1-AP (PTPRS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31720-1-AP targets PTPRS in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein
Full Name protein tyrosine phosphatase, receptor type, S
Calculated Molecular Weight217 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberNM_002850
Gene Symbol PTPRS
Gene ID (NCBI) 5802
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein tyrosine phosphatase receptor type S (PTPRS, also known as R-PTP-S, PTPSIGMA, and R-PTP-sigma) is an integral membrane protein. It can promote oligomerization by binding to large heparan sulfate proteoglycan structures, and interact with NTRK1/3, PPFIA1/2/3 (PMID: 25385546; 8524829; 9624153). The non-glycosylated molecular weight of PTPRS is 214 kDa, and the extracellular domain can be cleaved near the cell membrane by an endoprotease to generate a glycated 140 kDa protein. Variations and mutations of PTPRS are associated with Alzheimer's disease and cholangiocarcinoma (PMID: 38766183; 35991009).

Protocols

Product Specific Protocols
WB protocol for PTPRS antibody 31720-1-APDownload protocol
IHC protocol for PTPRS antibody 31720-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols