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CDX2 Polyclonal antibody

CDX2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30380-1-AP

Synonyms

CDX3, CDX-2, CDX 3, Caudal-type homeobox protein 2, Caudal type homeobox protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDX2 Polyclonal antibody (30380-1-AP)

    WB analysis using 30380-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30380-1-AP (CDX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CDX2 Polyclonal antibody (30380-1-AP)

    IHC staining of human colon cancer using 30380-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30380-1-AP (CDX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CDX2 Polyclonal antibody (30380-1-AP)

    IHC staining of human colon cancer using 30380-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30380-1-AP (CDX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using CDX2 Polyclonal antibody (30380-1-AP)

    IHC staining of mouse colon using 30380-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30380-1-AP (CDX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using CDX2 Polyclonal antibody (30380-1-AP)

    IHC staining of rat colon using 30380-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 30380-1-AP (CDX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CDX2 Polyclonal antibody (30380-1-AP)

    IHC staining of human colon cancer using 30380-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30380-1-AP (CDX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CDX2 Polyclonal antibody (30380-1-AP)

    IHC staining of human colon cancer using 30380-1-AP

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 30380-1-AP (CDX2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using CDX2 Polyclonal antibody (30380-1-AP)

    IF Staining of Caco-2 using 30380-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (30380-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

Tested Applications

Positive WB detected inmouse colon tissue, pig colon tissue
Positive IHC detected inhuman colon cancer tissue, mouse colon tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30380-1-AP targets CDX2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDX2 fusion protein Ag32826
Full Name caudal type homeobox 2
Calculated Molecular Weight 313 aa, 34 kDa
Observed Molecular Weight 33-40 kDa
GenBank Accession NumberBC014461
Gene Symbol CDX2
Gene ID (NCBI) 1045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.

Protocols

Product Specific Protocols
WB protocol for CDX2 antibody 30380-1-APDownload protocol
IHC protocol for CDX2 antibody 30380-1-APDownload protocol
IF protocol for CDX2 antibody 30380-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols