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VAV3 Polyclonal antibody

VAV3 Polyclonal Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 30291-1-AP

Synonyms

VAV 3, Guanine nucleotide exchange factor VAV3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VAV3 Polyclonal antibody (30291-1-AP)

    WB analysis using 30291-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30291-1-AP (VAV3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using VAV3 Polyclonal antibody (30291-1-AP)

    IP experiment of Jurkat using 30291-1-AP

    IP result of anti-VAV3 (IP:30291-1-AP, 4ug; Detection:30291-1-AP 1:8000) with Jurkat cells lysate 1560 ug.

  • Flow cytometry (FC) experiment of Jurkat cells using VAV3 Polyclonal antibody (30291-1-AP)

    FC experiment of Jurkat using 30291-1-AP

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human VAV3 (30291-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inJurkat cells, THP-1 cells
Positive IP detected inJurkat cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

30291-1-AP targets VAV3 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VAV3 fusion protein Ag33022
Full Name vav 3 guanine nucleotide exchange factor
Calculated Molecular Weight98kd
Observed Molecular Weight 98 kDa
GenBank Accession NumberNM_006113
Gene Symbol VAV3
Gene ID (NCBI) 10451
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vav3, a member of Dbl family which is also the activator of GTP kinase of Rho family, can activate the expression of RhoA, Racl and Cdc42 (PMID:28285969). Vav3 is associated with tumor growth, apoptosis, invasion, metastasis and angiogenesis (PMID:28285969). Vav3 is a multiple function protein with both signaling molecule and coactivator activities. Vav3 oncogene is overexpressed in androgen-independent prostate cancer cells relative to their androgen-dependent counterparts and contribute to development of hormone refractory prostate cancer (PMID:21455584).

Protocols

Product Specific Protocols
WB protocol for VAV3 antibody 30291-1-APDownload protocol
IP protocol for VAV3 antibody 30291-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nat Commun

Neutrophil extracellular traps-inspired DNA hydrogel for wound hemostatic adjuvant

Authors - Rui Ye
View Article