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LRRC50 Polyclonal antibody

LRRC50 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 30156-1-AP

Synonyms

DKFZp434A119, DNAAF1, FLJ25330, LRRC50

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using LRRC50 Polyclonal antibody (30156-1-AP)

    IHC staining of mouse testis using 30156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30156-1-AP (LRRC50 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using LRRC50 Polyclonal antibody (30156-1-AP)

    IHC staining of mouse testis using 30156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30156-1-AP (LRRC50 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using LRRC50 Polyclonal antibody (30156-1-AP)

    IF Staining of mouse testis using 30156-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using LRRC50 antibody (30156-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using LRRC50 Polyclonal antibody (30156-1-AP)

    IF Staining of mouse testis using 30156-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using LRRC50 antibody (30156-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30156-1-AP targets LRRC50 in IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LRRC50 fusion protein Ag32822
Full Name leucine rich repeat containing 50
Calculated Molecular Weight 725 aa, 80 kDa
GenBank Accession NumberBC024009
Gene Symbol LRRC50
Gene ID (NCBI) 123872
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRRC50 is a cilium-specific protein required for the stability of the ciliary architecture, it plays a role in cytoplasmic preassembly of dynein arms.

Protocols

Product Specific Protocols
IHC protocol for LRRC50 antibody 30156-1-APDownload protocol
IF protocol for LRRC50 antibody 30156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols